Analyzing which proteins - MS-TOF - Analyse results

5 important questions on Analyzing which proteins - MS-TOF - Analyse results

What is the program used for analysing ms?

MASCOT

That is tandem mass spectrometry (ms/ms)?

If the peptides are to big to be analysed for mascot you cut one up completely and run it to the ms again and now you will now which one it is.

What does SILAC stand for?

Stabel isotope labelling.
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How does SILAC work.

One proteins is build up with a light isotope (healthy person) and one with a heavy isotope (unhealthy person). You purify the sample and solate the protein, digest it and put it in the ms. Now you will now if there is a difference in the protein amount of a healthy or unhealthy person.

What does ICAT stand for?

Isotope code affinity tag.

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