Protein seperation - Protein tagging system

3 important questions on Protein seperation - Protein tagging system

Why do you use a protein tagging system?

To analyse quanternary sturctures of a protein. To see how protein complexes work.

How does a protein tagging system work?

You tage the protein that you wand to research with a TAG and put it back into the mixed protein solution. Then you run in trough a plate and the TAG wil bind to the surface. Becease the protein is interacting with other protiens the other protiens will get stuck to. You can separate these protiens and analyse them further trough ms.

What kind of short tags do you have?

His 6
Flag: specific dna segment
Shosrt dna segment: 3D structure similar to biotin

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