Microbial Evolution and Systematics - Molecular Phylogeny: Making sense of molecular Sequences
10 important questions on Microbial Evolution and Systematics - Molecular Phylogeny: Making sense of molecular Sequences
What is microbial systematics, and how does it combine phenotypic and genotypic information?
How does systematics link phylogeny with taxonomy, and what approach do taxonomists use to describe microorganisms?
What role do molecular sequences play in building phylogenetic trees, and why are small subunit ribosomal RNA (SSU rRNA) genes crucial in microbial phylogeny?
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Explain the process of obtaining DNA sequences for microbial phylogeny, emphasizing the role of PCR.
How does sequence alignment contribute to phylogenetic analysis, and why is it crucial?
Differentiate between homology and sequence similarity in the context of phylogenetic analysis.
What are orthologs and paralogs in the context of phylogenetic analyses?
How do phylogenetic trees represent evolutionary history, and what components make up a phylogenetic tree?
Explain the challenges and limitations of inferring phylogenetic trees, including the impact of homoplasy.
How is bootstrapping used to address uncertainty in phylogenetic trees, and what does a high bootstrap value indicate?
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