Microbial Growth and Its Control - Viable Counting of Microbial Cell Numbers
4 important questions on Microbial Growth and Its Control - Viable Counting of Microbial Cell Numbers
What is a viable cell?
What are two ways to perform a plate count?
- The spread-plate method
- The pour-plate method
What are limitations of plate counts?
- Culture media
- Incubation conditions
- Length of incubation
- Size of colonies
- Pure/mixed culture
Plate counting is subject to error for several reasons:
- Inaccurate pipetting
- Nonuniform sample
- Insufficient spreading
- Heat intolerance
- Biological variation
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What is better to express plate counting in than number of viable cells?
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