RNA sequencing - RNA-Seq Data
3 important questions on RNA sequencing - RNA-Seq Data
Uses of RNA-seq data in cancer genomics
- Expression levels (differential analysis)
- Variant calling
- Chimeric transcript detected (gene fusion)
- Tumor classification
Gene fusions can be detected with?
SNP microarray, karyotyping, FISH
Key points about RNA (gene fusions)
- Only detects functional chimeric events
- Requires heavy filtering due to false positives
- Is affected by the expression levels of genes involved
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