Sweetness and sweeteners - Positive allosteric modulators

5 important questions on Sweetness and sweeteners - Positive allosteric modulators

How can the bitter off-tastes or lingering of sweeteners be solved?

  • Combining sweeteners, so that their threshold does not exceed the negative effect
  • Positive allosteric modulators (PAMs)

How can the bitter off-tastes or lingering of sweeteners be solved?

  • Combining sweeteners, so that their threshold does not exceed the negative effect
  • Positive allosteric modulators (PAMs)

Where do PAMs bind?

They bind the same VFTD as the actual sweetener, but at non-overlapping sites. They are allosteric modulators.

  • Sweetener binds deep in the pocket of the VFTD close to the hinge region of the receptor domain
  • PAMs bind further away from the hinge region, stabilising closed conformation
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Can PAMs work in the absence of the sweetener?

No, they need pre-closure by the actual sweetener.

What are the most potent PAMs?

SE-2, SE-3 and SE-4

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