Enzym - carb - cellulase - xyla 0 pectinass

5 important questions on Enzym - carb - cellulase - xyla 0 pectinass

Hemi cellulose = xylan

Matrix of heteropolymers
backbone of beta 1,4 xylosyl units

potential side groups-
acetyl
arabinose
glucaronic acid

Primary and secondary cell wall

Drymmater more
1st fruits and veg - apple tomato
2nd   grains, seeds, wood - corn kernels ,straw

lot of pecting in primary
lot of xylan in 2nd

Secondary cell wall

Main:
1. Cellulose (fibrils) bweta 1,4 glucan chain
2 xylan (hemicellulose) - cover cellulose fibrils
3 lignin  (non-carb) is glue
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3 ebzymes for degradation of cellulose (beta 1-4 glucan)

Cellulases are hydrolases so use water
1. Endo-glucanases
2. Cellobiohydrolases (CBH) exo
! And 2
-active towards crystalline cellilose
-acts in synergy with endoglucanase
3. Celliobiase=beta glucosidase (to convert to glucose)
- inhibotr of CBH

Lytic polysaccharadie monoooxygenases

New class of enzumes

can cleave  in middle of cellulose, OXIDATIVE cleavage`, CAN HELP ENDOGLUCANASES

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