Enzym - carb - cellulase - xyla 0 pectinass
5 important questions on Enzym - carb - cellulase - xyla 0 pectinass
Hemi cellulose = xylan
backbone of beta 1,4 xylosyl units
potential side groups-
acetyl
arabinose
glucaronic acid
Primary and secondary cell wall
1st fruits and veg - apple tomato
2nd grains, seeds, wood - corn kernels ,straw
lot of pecting in primary
lot of xylan in 2nd
Secondary cell wall
1. Cellulose (fibrils) bweta 1,4 glucan chain
2 xylan (hemicellulose) - cover cellulose fibrils
3 lignin (non-carb) is glue
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3 ebzymes for degradation of cellulose (beta 1-4 glucan)
1. Endo-glucanases
2. Cellobiohydrolases (CBH) exo
! And 2
-active towards crystalline cellilose
-acts in synergy with endoglucanase
3. Celliobiase=beta glucosidase (to convert to glucose)
- inhibotr of CBH
Lytic polysaccharadie monoooxygenases
can cleave in middle of cellulose, OXIDATIVE cleavage`, CAN HELP ENDOGLUCANASES
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