Experimental procedures on animals
4 important questions on Experimental procedures on animals
Why are blood samples are take for animals:
- For analysis of biochemical, metabolic, toxicological or immunological parameters
- exmination or culture of micro-organisms
- production of antibodies
What is the total blood volume of animals?
- weight 25 g, so 1.8 ml
Rats have 64 ml/kg
- weight 300 g, so 19,2 ml
10% of total blood volume can be safely removed with fluid replacement (saline) of 15% (should be given subcateously and warm)
Why is the loss of blood volume not the main issue for multiple sampling?
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How can blood be sampled?
- Tail vein
- facial vein
- less clean as it comes in contact with fur
- orbite puncture
- eyes
- used on small rodents
- under anethesia
- clean blood --> directly
- less invasive due to no blood loss
- saphenous vein (leg)
- commonly used in rodents
- no anethesia
- can be clean (fur shaved)
- dorsal pedal vein
- paw
- small amount
- invasive
- marginal ear vein
- collection of blood in rabbits --> easy accessible and visible
- no anethesia
- cardiac puncture
- large amounts
- directly from heart
- always under anaesthesia
- only when terminating experiment
- exanguination
- maximum amount of blood
- decapitation or puncturing the aorta
- Cannulation
- producers where after painless and stress-less sampling can take place
- wel uitend for daily sampling
- discomfort only for the surcigy
- aseptic techniques
The question on the page originate from the summary of the following study material:
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