Summary: Developmental Genetics
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Read the summary and the most important questions on Developmental Genetics
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1 C. elegans Genetics
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What 2 concepts allow for a complete and exhaustive description of C. Elegans?
Simplicity and invariance -
C. Elegans development proceeds through?
Highly reproducible division patterns -
The formation and differentiation of each cell type can be followed and studied with?
Single-cell resolution -
Genetics starts with mutant alleles. Why isolate mutants?
- Genetic dissection of a biological process of interest
- Target selected mutagenesis (reverse genetics)
- Structure-function studies (missense mutation)
- Studie chromosomal aberrations (deficiencies and duplications)
- Genetic dissection of a biological process of interest
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What is the basic tool of geneticists to identify genes involved in a process?
Forward genetic screen -
Steps forward genetic screen
- Pick an interesting process
- Mutagenize animals/cells
- Identify mutants in which the process is disrupted
- Identify genes that are affected
- Pick an interesting process
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If during a genetic screen in C. Elegans the phenotype is lethal, what should you do?
Study the embryos -
2 Drosophila Genetics
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Advantages Drosophila as a model organism?
- Short life cycle
- Easy to edit genome
- No gene redundancy
- Easy to image
- Short life cycle
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How to find genes involved in a certain process?
Genetic screens -
After crossing, what should you study if homozygous mutants are not viable?
Score embryo/larvae for phenotypes
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