Drosophila Genetics

7 important questions on Drosophila Genetics

How would maternal-effect mutations be scored?

Score the progeny of living homozygous mutants

Why does the plasmid with the transposase not integrate into the DNA? (P-element)

It does not have 21bp repeats

P-element plasmid can integrate into the host genome and...

Stably pass on to the progeny
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Adult clonal screens

  • Screening mutant cells in a heterozygous adult animal
  • Maternal contributed protein is depleted at later stages
  • Using FlpFRT system to generate mitotic clones

Goal of P-element transformation

Insert a gene into a host genome

Goal Gal4-UAS

Targeted gene expression/knocking down of tissue-specific (ectopic expression) genes

Goal Gal4-Gal80

Targeted gene expression/knocking down of genes in a spatiotemporal manner

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