Jacques Botma - LlamaTags

6 important questions on Jacques Botma - LlamaTags

Goal of Jacques paper?

Demonstrate that the fluorescent protein tagging strategy reveals a role for inter-nuclear signaling and coordination of protein levels between neighboring nuclei in Drosophila embryogenesis.

Why is it hard to visualize protein concentration dynamics based on GFP?

In many model organisms, such as the fly, fluorescent protein fusions are degraded much faster than the rate at which they mature and begin to fluoresce.

How does LlamaTag overcome this slow maturation problem?

LlamaTags bind mature fluorescent protein and enable live imaging of TFs by leveraging fluorophore localization. The fluorescent protein is maternally produced, ensuring that all proteins are fluorescent and uniformly distributed throughout the embryo.
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How does LlamaTag work?

Upon translation of a LlamaTagged TF, the nanobody binds the fluorescent protein. Here, eGFP is translocated to the nucleus because of the Tfs nuclear localization signal, resulting in the enrichment of nuclear fluorescence.

What process might be important for coordinating gene expression to delineate straight and sharp boundaries of gene expression?

Inter-nuclear signaling

With the use of LlamaTags, Jacques showed that the concentration of protein in a given nucleus heavily depends on?

Transcription of that gene in neighboring nuclei.

The question on the page originate from the summary of the following study material:

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