Summary: Disease Diagnostics And Therapeutics
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Hypertrophic Cardiomyopathy ECGs, Immunohistochemistry
This is a preview. There are 14 more flashcards available for chapter 13/11/2018
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What are the 3 advantages of direct immunohistochemistry?
1. More specific method
2. Involves fewer steps
3. Useful when antigen is abundant -
What are the 3 advantages of indirect immunohistochemistry?
1. More sensitive method
2. Allows amplification
3. Useful when antigen is in low abundance -
Describe the structure of a direct immunohistochemistry
1. Antigen of interest fixed
2. Primary AB added to bind to antigen
3. AB has conjugated reporting system -
What is the conjugated reporting system for immunohistochemistry and what is its function? Give 2 examples
An enzyme that produces colour in presence of substrate - Shows presence and cellular location of antibody binding
E.g. Horseradish peroxidase enzyme/tagged with fluorophore -
Describe the structure of an indirect immunohistochemistry
1. Antigen of interest fixed
2. Primary antibody added to bind to antigen
3. Secondary antibody from a different species with conjugated fluorescence against primary antibody added -
What is diagnostic sensitivity?
The proportion of people with the disease who produce a positive test - The ability of the test to correctly identify patients with disease -
What is the equation for diagnostic sensitivity/PPV?
True positive/(True positive+False Negative) -
What are the 5 components required for PCR?
Buffer, DNA template, dNTPs, Primers, TAQ polymerase -
Sepsis
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When does CRP appear?
Hours after damage or infection -
What is the function and mechanism of action of CRP?
It binds to phosphorylcholine on bacterial cell surfaces - This fixes complement and promotes phagocytosis
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