Book summary - principles of genetic mainpulation
3 important questions on Book summary - principles of genetic mainpulation
How is gene silencing done?
- Making an gene knockdown by supressing at the RNA level.
- Antisense RNA: complement RNA (transgene) that can bind to the RNA so it cannot be synthesised, and functions of the protein stops.
- RNA interference (RNAi): a natural cellular pathway wh
ere the formation of double-stranded RNA induces specific gene inactivation.
- RNA-induced silencing complex (RISC)
- Leads to degradetion
- RNA-induced transcriptional silencing (RITS)
- Leads to repressing of transcription
Intracytoplasmic sperm injection (ICSI)
a method used in infertility treatment where sperm heads are injected into the cytoplasm of the egg, has been adapted to produce transgenic mice.
Reverse genetics screen
you know the defined gene/genes, and you need to create an abnormal phenotype that provides information on how gene(s) normally function. (can be genome editing)
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