Experimental measures of enzyme activity - Factors effecting the velocity of enzymatic reactions

5 important questions on Experimental measures of enzyme activity - Factors effecting the velocity of enzymatic reactions

What are the factors that effect velocity?

Enzyme concentration
pH effect
Temperature effect
Viscosity

At which pH is the enzymatic reaction optimal?

The pH that is close to the pKa of the enzyme

What are the different things you can measure with a change of pH?

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What ist he effect of temperature on the enzyme activity?

Higher temp will increase enzyme activity (more changes of substrate en enzyme bumping into each other) Till a certain point. Then there will be protein denaturation.

What for effect will the viscosity have on the enzyme?

Increase viscosity gives a lower viscosity.
How thinker the fluid how slower the reaction.

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