Reversed inhibitors - Dose-Response Curves of Enzyme Inhibition

4 important questions on Reversed inhibitors - Dose-Response Curves of Enzyme Inhibition

Why do you measure the dose response curve?

To see the effect a dose of inhibitor has on the enzyme activity.

What is really important when you are measuring the IC50 for different inhibitors?

Make sure the substrate concentration is always te same.

What is the Cheng and Prusoff relationship?

When you wand to wand to measure the inhibitory potential of different inhibitors.
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What equation do you use for the partial inhibitors?

Not the dose respons curve, but a more general formula.

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