Two component systems
10 important questions on Two component systems
What kind of molecules are sensed by bacteria?
- Carbon/free energy sources (glucose, lactose)
- Electron acceptors (oxygen, nitrate)
What are the essential nutrients for bacteria?
- Ammonium
- Phosphate
How can metabolic regulation be achieved?
- By controlling the activity of an enzyme
- By controlling the amount of active enzyme
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Which configuration has the regulatory protein mostly?
What is regulation of transcription by positive control?
Explain regulation of transcription by negative control (repression)
What are the main regulators group?
- One component regulators (cytoplasma bacteria, the sense a molecule and bind this to promoters so transcription can start)
- Two component regulators
What are the four important domains of FNR?
- Helix-turn-helix DNA binding site
- RNA polymerase contact size
- alfa-helical dimerization domain
- Oxygen sensitive 4FE-4S cluster (senses oxygen)
When is there Lac expression?
Describe the two component regulators
- Transmembrane signal sensor (SS) or histidine kinase (HK)
- Response regu..........
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