Two component systems

10 important questions on Two component systems

What kind of molecules are sensed by bacteria?

  • Carbon/free energy sources (glucose, lactose)
  • Electron acceptors (oxygen, nitrate)

What are the essential nutrients for bacteria?

  • Ammonium
  • Phosphate

How can metabolic regulation be achieved?

  1. By controlling the activity of an enzyme
  2. By controlling the amount of active enzyme
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Which configuration has the regulatory protein mostly?

Dimer configuration

What is regulation of transcription by positive control?

These transcription activators sense an inducer and bind upstream of and make physical contact with RNA polymerase. As such they stabilize the transcription initiation complex.

Explain regulation of transcription by negative control (repression)

These transcription repressors bind downstream RNA polymerase upon binding an co-repression. They prevent transcription.

What are the main regulators group?

  • One component regulators (cytoplasma bacteria, the sense a molecule and bind this to promoters so transcription can start)
  • Two component regulators

What are the four important domains of FNR?

  • Helix-turn-helix DNA binding site
  • RNA polymerase contact size
  • alfa-helical dimerization domain
  • Oxygen sensitive 4FE-4S cluster (senses oxygen)

When is there Lac expression?

When glucose is absent and lactose is present. So there is an integration needed of environmental (lactose) and intracellular (cAMP) signals. CRP binds and LacI falls of.

Describe the two component regulators

Its made up of 2 proteins
  • Transmembrane signal sensor (SS) or histidine kinase (HK)
  • Response regu..........

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