Basic Techniques

4 important questions on Basic Techniques

What are the DNA techniques?

  • Isolation
  • Manipulation/cloning
  • ..
  • ..

How does PCR work?

  1. Heat to separate DNA strand
  2. Let DNA primer bind (lower T)
  3. DNA polymerase makes new strand


repeat cycle 20-40 times

Function real-time qPCR

To amplify and at the same time quantify DNA. DNA is quantified by binding of fluorescence or by binding with a probe what decreases the fluorscence intensity.
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What are the different protein techniques?

  • Purfication
  • Presence, purity
  • Production
  • Localization
  • Interaction parterns
  • .........

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