Detection of harmful epitopes in foods - Other testing Principles
3 important questions on Detection of harmful epitopes in foods - Other testing Principles
What is PCR testing?
Detection of DNA fragments by a repeated amplification procedure.
What are advantages and disadvantages of using PCR testing for allergen detection?
Advantages
Disadvantages
- Well developed technique
- Relatively easy
- Highly sensitive
Disadvantages
- Presence or absence of DNA is not directly related to the presence of the antigen
- DNA is relatively heat labile: processing will lead to false negatives
Why is T-cell testing not often used in antigen testing?
Human T-cells can be used in antigen testing.
- This test can only be used in specialised laboratories since it requires advanced facilities for cell culture and monitoring T-cell reactivity.
- Also, since the test works with live cells, severe restrictions apply to the solvents used for antigen extraction
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