Detection of harmful epitopes in foods - Quantitative analysis: the Enzyme Linked Immune Sorbent Assay
7 important questions on Detection of harmful epitopes in foods - Quantitative analysis: the Enzyme Linked Immune Sorbent Assay
Which 3 different ELISA are there?
- Direct competitive ELISA
- Indirect competitive ELISA
- Non-competitive sandwich ELISA
What is direct competitive ELISA?
What is the process of non-competitive ELISA?
- Antigen solution is incubated with the antibody coated solid phase, in the absence of competing antigens or antibodies
- The amount of antigen bound is detected using a second enzyme conjugates antibody (Ig-PO)
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How long does direct competitive ELISA take?
What is an advantage of indirect competitive ELISA?
What is an advantage of non-competitive sandwich ELISA?
What is a disadvantage and advantages of ELISA?
- Not portable
Advantages:
- Instrumentation requires is relatively simple
- Analysis times can be optimised down to 20-30 min, by adjusting the concentrations of reactants used
- The procedure can be completely automated
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