Detection of harmful epitopes in foods - Quantification and calibration

4 important questions on Detection of harmful epitopes in foods - Quantification and calibration

Why is a level of quantification (LQD) required?

The LOD could be outside the linear range of the method.

LOD is always lower than LQD. T/F

True

What are the 2 basic requirements for quantification?

  1. The analysis protocol should include proper calibration to allow transforming raw data into amounts of allergen detected
  2. The analysis protocol should contain proper standards serving both as an internal control and a means of comparing between tests and between labs.
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What are collaborative studies or 'ring'-tests?

Check-samples are commercially available. These come with a subscription to a service in which laboratories can check their own data against those of others.

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