Practicals - Pre-lab assignment
39 important questions on Practicals - Pre-lab assignment
Bradford can measure the number of epitopes in the mixture. T/F
Bradford can measure the total amount of protein in the product. T/F
Bradford can measure the soluble protein content in the mixture. T/F
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Bradford is quantitative. T/F
Coomassie Brilliant Blue binds to the epitopes of proteins, therefore you can detect different epitopes. T/F
Why is it a problem that DNA is heat labile in PCR tests?
Presence or absence of a postive PCR outcome is not directly related to the presence of the antigen. T/F
PCR method is easy to perform at home. T/F
As outcome of the PCR method, you know if a specific sequence of DNA is present. T/F
In SDS-PAGE conformational epitopes are lost. T/F
SDS-PAGE is qualitative. T/F
SDS-PAGE gives an indication of the protein composition. T/F
Western blotting can detect linear epitopes. T/F
Dot blot detection is faster because no SDS-PAGE procedure is necessary. T/F
Dot blot detection is more specific than Western blotting. T/F
Conformational epitopes are also considered in the dot blot, while they disappear during the procedure for western blotting. T/F
Dot blotting does not provide information regarding the size of your protein. T/F
If you have an antibody with only one epitope, the non-competitive sandwich assay is not possible. T/F
The antigen of interest competes with a known amount of enzyme-labelled antigen to bind to the antibody. Which ELISA?
T-cell testing is a quantitative method. T/F
The outcome of mass spectrometry can indicate which protein was present. T/F
Bradford uses antibodies. T/F
PCR uses antibodies. T/F
SDS-PAGE uses antibodies. T/F
ELISA uses antibodies. T/F
T cell test uses antibodies. T/F
PCR gives a colour as result. T/F
SDS-PAGE gives a colour as result. T/F
Dot blot gives a colour as result. T/F
Dip stick gives a colour as result. T/F
Mass spectrometry gives a colour as result. T/F
PCR can detect conformational epitopes. T/F
Western blot can detect conformational epitopes. T/F
Dip stick can detect conformational epitopes. T/F
Mass spectrometry can detect conformational epitopes. T/F
Bradford is quantitative. T/F
ELISA is quantitative. T/F
Dip stick is quantitative. T/F
T cell test is quantitative. T/F
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