Summary: Genetic Studies Of Protein Function | Clarke

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• 1 Genetic studies of protein function

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• Open vraag: Give the definition of reverse genetics.

  Targeted disruption or interference of a gene so that the affect (if any) on the organism can be observed.

• Open vraag: Name 2 advantages of transgenic systems replacing chemical sprays.

  1: Partial supression of gene expression/ protein content. 

  2: Usefull for genes in multigene families. (Cosupress homologous genes simultanously).

• Open vraag: Name 2 dis-advantages of transgenic systems replacing chemical sprays.

  1: Supression is highly variable (can vary from plant to plant, generation to generation).

  2: Need to study 2-3 independent lines showing similar phenotype.

• Open vraag: Why do you need to study 2-3 independent lines showing similar phenotype.transgenic systems replacing chemical sprays?

  Overcome posibility of phenotype being due to TDNA instertion of antisense construct.

• Open vraag: The level of which hormone is higher in delayed senescence?

  The level of endogenous cytokinin.

• Open vraag: Name 2 advantages of delayed senescence.

  1: Changes in phenotype due to increased protein content /gene expression might be more informative than antisense repression.

  2: Usefull for genes in multigene families. 

• Open vraag: Why is delayed senescence usefull for genes in multigene families?

  Because it helps you distinguish possible differences in function between members.

• Open vraag: Name 2 dis-advantages of delayed senescence.

  1: Increased protein content is highly variable. (Can vary from plant to plant, generation to generation).

  2: Can lead to gene silencing by decresing protein inhibition. 

• Open vraag: With what kind of RNA are plants transformed (DS or SS) and to what mRNA do they need to have a sequence similarity?

  DSRNA. They need to have a sequence similarity to targeted mRNA.

• Open vraag: With what protein complex is small interfering RNA (siRNA) associated? And what is the meaning of this abreviation?

  With RISC. RNA Induced Silencing Complex.