DNA replication - Eukaryotic DNA replication
20 important questions on DNA replication - Eukaryotic DNA replication
The assembly of which complex is needed for DNA replication in eukaryotes?
What acts as the first initiator of preRC assembly?
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To what does the binding of MCM helicase to the leading strand complete?
Which of the proteins of the prereplication complex (preRC) do also play a role in the elongation?
Which 3 types of DNA polymerase play a role in DNA replication within the cell nucleus?
What does DNA polymerase alfa do?
What happens after the primer is made by DNA polymerase alfa and primase?
How is the exchange DNA polymerase alfa to DNA polymerase epsilon or sigma, called?
What is the role of DNA polymerase beta?
What are translesion-replicatiing polymerases?
How are the RNA primers removed in bacteria? And in eukaryotes?
- Eukaryotes: flap endonuclease.
What causes the flaps in primers (figure 11.21)?
Why are telomeric repeat sequences needed?
What happens when the problem of the inability of DNA polymerase to replicate the 3' end is not solved?
How is this problem solved?
In which three phases does telomere lengthening occur?
2. Polymerization: the RNA sequence beyond the binding site functions as a template for the lengthening of the 3' overhang;
3. Translocation: every time telomerase moves further and adds new nucleotides to the 3' end.
Which subunits of telomerase catalyze telomere lengthening?
What happens when people age?
How do cancer cells keep dividing when telomeres become shorter?
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