Translation of mRNA - Experimental determination of the genetic code
5 important questions on Translation of mRNA - Experimental determination of the genetic code
Which three research groups played a role in deciphering the genetic code?
- Group headed by Ochoa
- Group headed by Khorana
What is a cell-free translation system?
When the percentages of added nucleotides are known, how can the relative amounts of the possible codons be predicted?
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How was the experiment of Nirenberg and colleagues set up?
- They added a mRNA polymer of 70%G and 30%U;
- They calculated the relative amount of the possible codons;
- They added amino acids; one was radiolabled;
- They waited 60 min for transcription to occur;
- They looked at the %s of radiolabled amino acid into the translated polymers and compared it with the calculated relative amounts of polymers to determine which codon results in a certain amino acid.
Khorana and colleagues performed a similar experiment. What did they different then Nirenbergs experiment?
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