Mesoderm & skeleton

30 important questions on Mesoderm & skeleton

When does mesoderm formation take place, and from what does it develop?

- In week 3 and 4
- Epiblast cells form between epiblast and hypoblast to form intraembryonic mesoderm.

What controls the mesoderm cell-specification?

FGF-8.

Where do the different muscle types develop from?

- Skeletal muscles develop from paraxial mesoderm
- Smooth muscles:
  • in gut and derivatives: develop from visceral layer of lateral plate mesoderm
  • pupil, mammary and sweat glands: ectoderm
- Cardiac muscles develop from visceral layer of lateral plate mesoderm
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How does the process of somite differentiation go?

- At first, somites are epithelial and have a lumen
- The ventral and medial part become mesenchymal, migrate around the neural tube and notochord (sclerotome)
- The dermamyotome forms
- Dorsomedial and ventrolateral cells become muscle cells
- The cells migrate medial and ventral

Which trancription factors play a crucial role in this process of somite differentiation?

BMP4 and Wnt
- At the dorsomedial side, there are medium levels of BMP4 and high levels of Wnt
- At the ventrolateral side, there are high levels of BMP4 and medium levels of Wnt

What does the gradient of BMP4 and Shh also induce?

Expression of other factors, like PAX genes.

What are PAX genes important for?

- Determine the fate of neural cells (dorsal-ventral specification)
- Development of dermatome

What is the role of Shh and FGF in somite differentiation?

- It upregulates chordin and noggin;
- which cause the ventral part of the somite to become sclerotome

What is the role of PAX3 in somite differentiation?

It is also expressed in the dermatome, and together with NT3 regulates it the dermatome -- dermis formation

What is the role of MYG5 in somite differentiation?

Together with Wnt it specifies cells into dermamyotome (muscle formation).

What is the role of MYOD in somite differentiation?

It is the second group of muscle cell precursors (lowest picture: expression of MYOD is in the part indicated with 'VLL')

Why is there a split and combine in the sclerotome formation?

It has to make a way for the nervous system, the muscles need to be innervated.

What does each somite induces?

The outgrowth of a spinal nerve.

How many sclerotome fuse in this process, and how many vertebrae are formed with this process?

- 8x sclerotomes before
- 7x vertebrae after

What does the notochord contribute to?

To the formation of the nucleus pulposes (which gives structural strength in young children)

What kind of division can be made between the thoracic ribs?

  • True ribs (1-7) connect to the sternum via their own cartilage
  • False ribs (8-10) attach to the sternum via cartilage of other ribs
  • Floating ribs (11-12) do not connect to the sternum

How is proved that cell fate in the to-be rib cells is already determined?

By transplantation experiments.

What are the three categories of defects on the spinal vertebrae?

  • Congenital: process of splitting and fusion went wrong
  • Idiopathic: unknown origin
  • Neuromuscular: as a result of another disease

What nerves that run through the openings in the vertebrae innervate the muscles?

The ventral root neurons.

What are the peripheral nerves that pass through the openings of the vertebrae?

Dorsal root ganglia, which are sensory neurons

Why are the dorsal root ganglia not considered part of the CNS?

Their cell bodies are out of the vertebrae column.

What are the two components of the skull?

  • The neurocranium
  • The viscerocranium

picture: viscerocranium

What does the neurocranium consist of?

  • Membranous part (flat bones that surround the brain)
  • Cartilaginous part (forms bones of) the base of the skull

What does the membranous neurocranium consist of?

  • Neural crest cells
  • Paraxial mesoderm

Mesenchym from neural crest cells and paraxial mesoderm undergo ossification without making cartilage.

What is the membranous neurocranium typified by?

By the bone spicules that radiate outwards.

How does the cartilaginous neurocranium form?

Initially it forms cartilage, which fuse into 3 chordocranium types.

What does the growth of the skull depend on?

On the growth of the brain (so microcephaly originally is a growth retardation of the brain, not of the skull)

How are the separate flat bones of the skull connected to each other?

With seams of connective tissue (act as sutures9

Where are the bones of the face formed from?

From the neural crest cells of the first two pharyngeal arches.

What are the three structures that form from the first two pharyngeal arches and give rise to the face?

  • Mandibular prominence
  • Maxillary prominence
  • Nasal prominence

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