Tissue preparation & techniques
17 important questions on Tissue preparation & techniques
In which position should you place the condenser for a stained and unstained slide?
- unstained slight condenser in lowest position ( to get more contrast)
- stained in highest position
Where can we find the artery arcuate and which kind of tissue does it consist of?
Is there shrinkage of tissue during drying?
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A human kidney slide with 20 glomeruli will show at least 15 planes with a tubular pole, true or false?
Is it normal to see a capillary loop in the lumen of a proximal tubule?
Can a slide of solitary cells (puncture or smear) provide the same information as an tissue (histology) slide?
What is the function of fixation of tissue
For a good image you need a high antigenicity, how can this be achieved?
Antigenicity = describes the ability of a foreign material (antigen) to bind to, or interact with, the products of the final cell-mediated response
Which slides have better resolution? Thinner or thicker slides?
But the activity (of proteins?) is much higher in a thicker slide (and resolution lesser)
Eosin and ... Hematoxylin (HE stain) are most common used staining, what do you stain?
Connection of GBM and bowman's capsule means?
Why do we see more with microscopic microscope then with light microscope?
Resolution = K x wavelength / NA (effe checken)
so green light has a better resolution than red light, because green has a shorter wave length.
Shorter wave length = smaller R = better resolution
With a cryo fixation the antigenicity will improve, true or false?
What is the real disadvantage of cryo fixation?
good solution but also has disadvantage (expensive etc) is the below:
High pressure freezing (at higher pressure water will not freeze at 0 C but at -40 c (zoiets??) so you have much more time. Nice to get a high immune histochemistry and thus high antigenicity.
What do we detect with CLSM (confocal laser scanning microscopy)?
when the pinhole is increased in CLSM it turns in a normal standard fluorescence microscopy, because not only what is sharp is shown anymore
What can we measure with EDX = X-ray scanning microscopy (orzo?)
So you can only measure elements but not the structural formula of a substance.
Which tissue samples sorts are there?
LM
EM
Specific (EM Pact-HPF)
Molecular Applications
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