Laboratory techniques - Isolation of an antigen
3 important questions on Laboratory techniques - Isolation of an antigen
What are the steps in immunoprecipitation?
2. Add excess immobilised antibody specific for antigen of interest
3. Collect immobilised antibody by centrifugation
4. Wash with fresh solution to remove unbound antigens
5. Denature antibody to elute antigen
What is affinity chromatography used for?
What are the steps in affinity chromatography?
2. Add solution with mixture of antigens
3. Wash away unbound antigens
4. Elute antigen X
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