MHC predictions
5 important questions on MHC predictions
What are the differences between HIV progressors and controllers?
- Progressors
o High copy number
o Causes AIDS
o ART suppressed (antiretroviral therapy)
- Controllers
o Much lower concentration
o No ART required
What is/are the best determint(s) of HIV-1 disease progression?
Rate of HIV disease progression strongly associated with expression of particular HLA-B alleles.
What an how do proteins of HIV-1 virion correlate with the viral load?
Gag responses give a negative correlation lower viral load
Env responses give a positive correlation higher viral load
- Higher grades + faster learning
- Never study anything twice
- 100% sure, 100% understanding
How do we know which peptides bind to an MHC molecule?
1. Obtain purified HLA or recombinant heavy chain & β2m
2. Obtain indicator peptide (reporter)
3. Perform dose titration of any inhibitory peptide
4. Separate free from bound peptide
5. Calculate binding and IC50 --> lower IC50, better binding
How do we decide which are good binders and thus epitopes?
Use a fix threshold, and decide that all the
peptides binding better than this threshold are likely epitopes. Take top 1-5% of all possible 9mers as possible epitopes. When comparing peptide binding to different HLA
molecules: fix thresholds do NOT work! Use ranks
instead!
The question on the page originate from the summary of the following study material:
- A unique study and practice tool
- Never study anything twice again
- Get the grades you hope for
- 100% sure, 100% understanding
![Trustpilot Logo](/images/design_2020/lp/clp-trustpilot-en.png)