Mapping Eukaryote Chromosomes by Recombination
5 important questions on Mapping Eukaryote Chromosomes by Recombination
What cellular process produces a recombination of linked genes?
Linkage explains why the parental gene combinations stay together but not how the recombinant (nonparental) combinations arise. Morgan postulated that, in meiosis, there may be a physical exchange of chromosome parts by a process now called crossing over. A result of the physical breakage and reunion of chromosome parts, crossing over takes place at the four-chromatid stage of meiosis. Thus, there are two types of meiotic recombination. Recombination by Mendelian independent assortment results in a recombinant frequency of 50 percent. Crossing over results in a recombinant frequency generally less than 50 percent.
How are recombination maps used in conjunction with physical DNA maps?
In genetics generally, the recombination-based map of loci conferring mutant phenotypes is used in conjunction with a physical map such as the complete DNA sequence, which shows all the genelike sequences. Knowledge of gene position in both maps enables the melding of cellular function with a gene’s effect on phenotype.
three-point testcross (three-factor testcross)
three genes linked and, if they are, to deduce their order and the map distances between them
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Coefficient of coincidence (c.o.c.)
quantification of interference(I) by first calculating c.o.c., which is the ratio of observed to expected double recombinants.
I=1-c.o.c.
simple sequence length polymorphisms
simple sequence length polymorphisms (SSLPs), repeats of DNA, unclear what the function is.
Also known as variable number tandem repeats (VNTRs).
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