Enzymes and metabolic pathways - Factors affecting enzyme activity
4 important questions on Enzymes and metabolic pathways - Factors affecting enzyme activity
How does higher temperature influence chemical reactions?
Molecules move faster
Easier for electrons to gain activation energy
What are other effects of temperature on protein?
Denaturation; irreversible loss of activity
Protein chain unfolds; active site is lost
Becomes insoluble and precipates out of solution
What substrates are mostly involved in enzyme-catalyzed reactions?
Catalyzing lysis of a molecule
Isomerization reaction (only a single substrate)
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What is meant by cooperativity in cooperative (allosteric) enzymes?
Cooperation of subunits of the complete enzyme by binding the substrate to active site (which changes the conformation of the whole multisubunit arry), affecting other active sites altering the ease which the substratecan bind. Also allesteric enzymes due to the change in the conformation (shape).
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