Hematopoietic stem cells (Robin)
8 important questions on Hematopoietic stem cells (Robin)
Two ways of identification of mature hematopoietic cells
- Morphology
- Flow cytometry analysis (after staining with antibody marker)
Two ways of identification of hematopoietic progenitors
- Clonogenic assay (CFU-C)
- Formation of colonies into semi-solid medium supplemented with cytokines and serum.
- Complex multi-parameter flow cytometric isolation
Two ways of identifying HCSs in vivo
- PCR
- Flow cytometry
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When and where are the first HSCs produced during development?
Is the process of HSC formation conserved between species?
Where do HSCs and clusters come from?
What is the exact composition of the clusters?
- Clusters are formed by immature pre-HSCs that progressively mature into HSCs.
- 700 cluster cells
- 3 ESC per aorta
How to identify genes expressed around the aorta at specific time points and locations?
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