16s RNA analysis

3 important questions on 16s RNA analysis

What is 16s RNA analysis?

Ribosomal RNA, universally present in all bacteria;
Sequence based phylogeny marker;
Conserved regions for PCR primers for amplification;
Variable regions to recognize specic bacterial species/OTUs.

What are the components of bacterial phylogeny?

Phylum: Firmicutes
Class: Bacilli
Order: Lactobacillales
Family: Streptococcaceae
Genus: Streptococci
Species/OTU: S. thermophilus

What are characteristics of our microbiota?

80% in 3 major phyla: Bacteroidetes, Firmicutes, Actiniobacteria
Stable over time
Resilient
Subject-specific

The question on the page originate from the summary of the following study material:

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