16s RNA analysis
3 important questions on 16s RNA analysis
What is 16s RNA analysis?
Ribosomal RNA, universally present in all bacteria;
Sequence based phylogeny marker;
Conserved regions for PCR primers for amplification;
Variable regions to recognize specic bacterial species/OTUs.
What are the components of bacterial phylogeny?
Phylum: Firmicutes
Class: Bacilli
Order: Lactobacillales
Family: Streptococcaceae
Genus: Streptococci
Species/OTU: S. thermophilus
What are characteristics of our microbiota?
80% in 3 major phyla: Bacteroidetes, Firmicutes, Actiniobacteria
Stable over time
Resilient
Subject-specific
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