Microbial diagnostics
6 important questions on Microbial diagnostics
How can bacteria be detected?
Detection of agent
- microscopy
- Methyleen blue colours gram+ bacteria
- culture
- Incubation: environment, time depends by material and clinical image
- PCR (nucleic acid detection)
- Not/difficult to culture
- quick
- antigen detection
Serology: Detection of immunoglobulins against agent
What are the different types of culture media?
- Selective: specific for one type of bacteria
- Non-selective: blood culture lots of nutrients
- Sterile (liquor, usually no bacteria in there - blood culture)
- Non-sterile (feces, specific culture due to lots of bacteria present)
What are the bacterial identification methodes?
- Biochemical (bonte rij, Vitek, Phoenix)
- MALDI-TOF MS
- Sequence analysis of bacterial DNA
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How is the antibiotica resistance determined?
- Resistance determination by the gold standaard (vroeger)
- at MIC = 125 µmol/l bacterie does not grow anymore
- Standardised and automised (now)
- Data base
- PCR of genome/plasmid of bacteria
What bacteria are used in PCR (nucleic acid detection)?
- MRSA
- SSYC
- Legionella
- Mycrobacteria
What does serologie show? Which bacteria are used in serologie?
- Kinkhoest (Bordetella pertussis/parapertussis)
- Burcella
- Helicobacter (antigen in feces)
- Legionella (antigen in urine)
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