Skin and biomaterial infection - Biofilms, antimicrobials and resistance
3 important questions on Skin and biomaterial infection - Biofilms, antimicrobials and resistance
How do bacteria attache to biomaterial surface?
- Bacterial attachment on protein-coated biomaterial surface through adhesion
- Biofilm formation through
- Exopolysaccaride production
- Release of DNA
- Factors of proteic nature
What are the steps to form biofilm?
- Primary attachment phase
- Aap and MSCRAMM bind the surface
- Accumulation phase
- Aap/SasG B domaine mediated accumulation and MSCRAMM mediated accumulation
Detachment/dispersion phase:
Phenol-soluble modulins (PSMs)
- strongly amphipathic alfa-helical peptides
- biofilm structuring and dispersal
- under quorum sensing control
- (‘Quorum Sensing’ (QS) to describe the phenomenon whereby the accumulation of signaling molecules enable a single cell to sense the number of bacteria (cell density))
What happens to bacteria within biofilm?
- Difficult to phagocytose
- Expressing different gene sets than planktonic
- Regulate gene expression by “quorum sensing”
- Produce extracellular polysaccharide increasing biofilm and/or biofilm dispersing molecules
- Not effectively reached by all antibiotics
- In “dormant state”, bacteria are less susceptible to antibiotics
- Persisting inflammatory stimulus
Local immunity is compromised, allowing intracellular survival
Biomaterial together with bacteria derange immune responses
The question on the page originate from the summary of the following study material:
- A unique study and practice tool
- Never study anything twice again
- Get the grades you hope for
- 100% sure, 100% understanding
Remember faster, study better. Scientifically proven.
