Genomes, OMICS and Bioinformatics
7 important questions on Genomes, OMICS and Bioinformatics
What is the definition of genome annotation?
Describe the annotation of a DNA sequence
- 5' end of gene
- regulatory region
- promotor (TATA box)
- transcription start site
- transcribed region
- coding sequence
- startcocon AUG
- Exon and intron
- terminator codon
- AAUAAA signal for 3' end trimming and polyAtail addition
- termination site
Which sequences are required for intron removal?
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What is differential and alternative splicing?
Which nucleotide changes can be subject to strong selection?
- no selection Ka/Ks > 1
- Ka = the non-synonymous substitution rate (base change leads to different amino acid)
- Ks = synonymous substitution rate (base change leads to same amino acid)
Explain homolog, ortholog and paralog
Ortholog = genes in different species that evolved from a common ancestral by speciation (same function)
Paralog = genes related by duplication within a genome (different function)
Which techniques are used for RNA Transcriptomics?
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