Transgenesis
9 important questions on Transgenesis
What is the use of transgenic cells?
What are Transfectan and TFX Reagent?
How is DNA transferred to anime cell culture?
- Directs DNA transfer (microinjectie)
- Transfection (chemical)
- Transduction (viral transfer)
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What are the difference between transient expresion and stable integration?
Stable integration is permanent and integration of vector DNA into genome
What is important to consider when doing transformation experiments?
Explain how transgenic mouse can be made
2. The ES cells are then injected in blastocyst
Why do pharmas use animal instead of bacteria to produce recombination pharmaceutical proteins?
How can you study the gene activity in transgenic animals?
- Gene replacement
- gene knockout
- gene addition
What is CRIPR/CAS9?
CRISPR–Cas9 uses a small strand of RNA to direct the Cas9 enzyme to a site in the genome with a similar sequence. The enzyme then cuts both strands of DNA at that site, and the cell's repair systems heal the gap.
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