Vesicular transport
8 important questions on Vesicular transport
What is the difference between mitochondrial signal peptide and the ER signal peptide?
Explain how SRP is used in ER targeting
- SRP binds to signal sequence of the rowing peptide
- binding causes a pause in translation
- SRP binds to the SRP receptor in ER membrane
- translation continues and translocation begins
How are ER proteins transported to the ER?
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What is the role of protein glucosylation?
- Protein N-glycosylation in the ER takes place on asparagine residue
- Proteins are glycosylated on asparagine residues
(N-linked glycosylation) & threonine (O-linkedglycosylation)
What is the role of COP and clathrin in vesicular transport?
- clathrin coats are needed to form the endosome vesicle
Explain how endosome vesicle are made?
- Cargo receptor proteins are recognized by AP and will bind (coat assembly and cargo selection)
- Cargo binds to receptor and Clathrin binds to the AP (bud formation)
- GTP hydrolysis in dynamin drives a conformational change and brings membran in close proximity in membrane fusion (vesicular formation)
- uncoating
What are the roles of RAB and SNARE?
What is the role of lysosomes?
- soluble lysosomal enzymes are decorated with mannose-6-phosphate by GlcNAc phosphotransferase
- Specific targeting of soluble hydrolases to lysosomes uses a pH dependent low Km ER M6P-receptor & vesicle transport
The question on the page originate from the summary of the following study material:
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