Mechanisms of Development - Tutorial - Extra vragen

5 important questions on Mechanisms of Development - Tutorial - Extra vragen

Multicellular organisms exhibit
a.
b.
Development requires mechanisms for:
c.
d.  
Do these requirements lead to striking similarities between plants and animals and an 'overall logic of development'?

a. Pattern formation (ie. Division of tasks)
b. Genomic equivalence

c. Cell-cell communication
d. Differential gene expression

yes

In which 6 steps can eukaryotic gene activity be controlled?

1. Transcriptional control
2. RNA processing control
3. RNA transport and localization control
4. Translation control
5. MRNA degradation control
6. Protein activity control

Wat zijn homeotic genes?

Genen die een rol spelen in identiteitsbepaling en ontwikkeling, deze genen coderen in de meeste gevallen voor transcriptiefactoren met homeodomein.
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Kunnen transcriptie factoren activator en inhibitor zijn?

Ja

How to quantify gene expression?

  • Northern Blotting
  • Quantitative Reverse Transcriptase PCR (qRT-PCR
  • Micro arrays
  • RNA sequencing (RNAseq)
  • Single cell RNAseq

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