Summary: Microbiology Lab
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1 Microbiology Lab
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Which contaminated Materials go into the following: White buckets, White buckets w/ orange liner, Benchtop (R2) units, Trash can
White Buckets: Test Tubes
White buckets with liner: Petri Plates
R2 units: Micoscope slides
Trash Can: None (Paper towels are not contaminated) -
What color will gram - organisms be at each step
Crystal Violet: Purple
Iodine: Purple
Decolorizing: Clear
Safranin: Red -
Why is a thick smear not ideal for staining
The organisms layer on top of each other, it is hard to see individual organisms -
What is a CFU? Why is it used?
CFU = Colony Forming Unit. It is used because it is never certain that a colony arose from a single bacteria cell as sometimes they clump together -
Why are agar plates incubated upside down?
So that condensation does not drip onto the plate, interfering with colony growth -
How does a capsule contribute to pathogenicity?
- Makes organism more hardy to environment
- Sometimes Inhibits immune response of host cell due to composition
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What feature does "negative staining" allow us to observe? Describe what this looks like.
Negative staining allows us to see the capsule of a bacterium. The bacteria itself is stained red, the background of the slide stains a blue color and the capsule itself retains no dye and appears as a clear negative. -
What techniques is used for the primary staining of a spore stain procedure? Why is this used?
The primary stain was applied onto a paper towel over a steam bath. The heat is necessary in order to allow the stain to penetrate the spore coat -
Know where the following are located on a microscope
Eyepiece
Nosepiece
Objective lens
Slide Clip
Stage
Condenser
Light brightness adjustment dial
Lamp
Coarse focus
fine focus
Stage controls -
Formula for resolving power
Wavelength / Numerical Aperture
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