Microbiology Lab
73 important questions on Microbiology Lab
Which objective lens is engaged during storage
Which bacteria contains a capsule
In a pour plate, why do the colonies on the surface of the agar appear larger than colonies inside of the agar?
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Acid Fast staining procedure binds to what part of the cell wall of acid fast bacteria?
True/False: Bacterial endospores may be detected by gram stain
Staphylococcus epidermidis is a Gram(+/-) Bacillus/Cocci
For a plate count to be st atistically significant, what range must the # of colonies be within
What objective lens requires oil?
The ocular lens of a compound microscope magnifies objects:
True/False: The caps on our incubation tubes should be left slightly loose during incubation
Which of the following can be used to isolate a colony: Agar slant, Streak plate, Nutrient Broth?
Match the following:
A. Contrast
B. Resolution
C. Magnification
1. Increases apparent size of object
2. Can be improved by staining
3. The ability to distinguish 2 closely spaced objects
2 : A
3 : B
What is the primary stain and counterstain for a Capsule Stain?
Counterstain: Maneval's stain
What is the primary stain and counterstain for a Spore Stain?
Counterstain: Safranin
What is the primary stain and counterstain for an Acid Fast Stain?
Counterstain: Methylene Blue
What is the formula for calculating Original Cell Density? (OCD)
T/F A Psychrophilic organism prefers very high temperatures
T/F Bacterial Endospores may survive in prolonged UV exposure
T/F An exoenzyme must be produced by a bacterium to digest monosaccharides
T/F The process of pasteurization is a form of sterilization
T/F The use of an autoclave for sterilization can destroy bacterial spores
T/F Joseph Lister was the first to introduce the concept of controlling bacteria with heat
T/F Staphylococcus aureus is a thermophilic organism
T/F A phenol coefficient refers to the effectiveness of a disinfectant
T/F A capnophile thrives in high levels of CO2
T/F The phenylalanine test is highly specific for Bacillus subtilis
In the phenylalanine test, ferric chloride is added to detect which endproduct of the reaction?
Describe the nitrate reductase test
In the nitrate reductase test, zinc is added to detect what?
Why can Bacillus subtilis survive under UV light?
The destruction of all microbial life is termed:
What method was used to limit the presence of Oxygen in the O/F test?
How does UV light damage an organism?
A chemical used to control bacterial growth on non-living tissues is a
What indicator is used in the sugar fermentation test?
What endproduct is detected by the Durham tube in the sugar fermentation test?
Mueller-Hinton agar is used for what test?
Why is Pseudomonas aeruginosa a true denitrifier?
What is the purpose of an exoenxyme?
Describe where an aerobe, a facultative anaerobe, and an anaerobe would grow in a thiglycallate test tube.
Facultative anaerobe: in both the top and bottom layers (everywhere)
Anaerobe: In the bottom layer only
For detection of the exoenzyme caseinase, what media is used, what reagents are required, and what does a positive test look like?
Reagents: None
Positive: Clearing of the cloudy color around the organism
For detection of the exoenzyme a-amylase, what media is used, what reagents are required, and what does a positive test look like?
Reagents: Iodine
Positive: Repelling of the iodine by the colonies
For detection of the exoenzyme DNase, what media is used, what reagents are required, and what does a positive test look like?
Reagents: None
Positive: Pink/purple color around organism
For detection of the exoenzyme Lipase, what media is used, what reagents are required, and what does a positive test look like?
Reagents: None
Positive: Clearing of turbidity (not color!)
For detection of the exoenzyme Gelatinase, what media is used, what reagents are required, and what does a positive test look like?
Reagents: None - cool in ice bath
Positive: liquid after cooling
How is the Kirby Bauer test performed and interpreted?
How would you experimentally determine the optimal growth temperature?
- Inoculate multiple test tuebs w/ an organism
- Incubate each at a specific temperature for a set amount of time
- Compare the turbidity of each tube to see at which point the most growth occurred
Which organism is catalase -?
Staphylococcus aureus is distinguished from other staphs by which characteristic
A and P disc sensitivity distinguish species of which bacterial family?
Indole can be detected in the culture of a bacterium that produces which enzyme?
The reason for using a candle jar to incubate streptococci is...
The stab marks in the hemoysis test allow us to detect Streptolysin O or Streptolysin S?
Streptolysin S is (inhibited/ stable) in oxygen
What 2 enzymes can break down hydrogen peroxide?
Peroxidase
What type of coagulase is detected by the rabbit plasma tube agglutination test?
Streptococcus pneumonia can be differentiated from other a-streptococci by antibiotic sensitivity to:
The sodium Hippurate test is useful in identifying which bacteria?
Which organism is Novobiocin resistant?
What reagent is used in the Sodium Hippurate test and what does it detect?
Detects Glycine (an amine)
T/F Gram+ organisms can grow on MacConkey agar if they are lactose fermenters
Gram - only
Grow bright pink if they are lactose fermenters
What tests can determine if a bacteria is lactose fermenting?
KIA test
What ingredients are in MacConkey agar
bile salts
lactose
neutral red
H2S can be detected by which 2 tests?
SIM
T/F The caps on citrate and urea tests must be left loose for incubation
Antibiotics in CNA agar are:
Nalidixic acid
What is determined by the methyl red test?
How do you read a KIA Test?
Bottom = glucose
Yellow = acidic = positive for fermentation
Red = alkaline = negative for fermentation
Black = H2S produced
What tests are each of the following media used for?
Mueller Hinton Agar
MacConkey Agar
CNA Agar
Tryptophan broth
Rabbit Plasma
MacConkey Agar - Gram - isolation
CNA Agar - Gram + isolation
Tryptophan broth - Indole production
Rabbit Plasma - Coagulase production
What reagents are used for the following tests?
Catalse
Na Hippurate
Indole
Vogues-Proskauer
SIM
A disc sensitivity
P disc sensitivity
Urea
KIA
Na Hippurate - Ninhydrin
Indole - Kovac's reagent
Vogues-Proskauer - a-napthol and potassium hydroxide (VPA and VPB)
SIM - Kovac's reagent
A disc sensitivity - Bacitracin
P disc sensitivity - Optichin
Urea - No reagent
KIA - No reagent
What media is used for the hemolysis test?
How is the hemolysis test innoculated?
Name an example of a bacteria exhibiting each type of hemolysis
B - Streptococcus pyogenes
y - Enterococcus faecalis
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