RNA isolation and quality control - Yeast cells an eukaryotic model

6 important questions on RNA isolation and quality control - Yeast cells an eukaryotic model

What is often considered as the model eukaryotic organism?

Saccharaomyces cerevisiae (baker's yeast)

What can you say about the cell wall of a yeast cell?

It is a polysaccharide and it needs to be remover for many types of procedures.

How do we call yeast cells without a cell wall?

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Where can yeast cells grow?

Generally in a rich medium (YPD medium, containing yeast extract, peptone and dextrose) when special conditions are not required.

How can the density of cells in a culture be determined?

By spectrometry, measuring its optical density (OD) at 600 nm.
For reliable measurements, cultures should be diluted such that the OD600< 1. In this range each 0.1 OD600 unit corresponds to ~
3 *  10^6 cells/ml

What is the difference between haploid and diploid cells?

Diplois refers to the number of complere chromosome sets present in each cell of an organism: diploid cells contain two complete sets.
Haploid organisms, on the other hand, only contain one complete chromosome set. Chromosomes sets can be altered in meiosis, and occasionally in mitosis.

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