Restriction digest of phage lambda DNA and Agarose gel elektrophoresis - Phage lambda DNA - Genome of phage lambda
3 important questions on Restriction digest of phage lambda DNA and Agarose gel elektrophoresis - Phage lambda DNA - Genome of phage lambda
During this lab session we work with this genome of the phage lambda. What does it consist of?
It consists of a double-stranded DNA-helix of 48502 base pairs in circular shape.
The COS-sites on both ends of the linear molecule are complementary to each other, what does that mean?
They can bind to each other ro form a circular molecule. The COS-sites are comparable with the sticky-ends of the some restriction enzymes.
What happens when a λ injects its DNA in a host?
It converts from linear to circular shape, in order to protect itself against degradation by the bacterial exonucleases of the host.
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