Restriction digest of phage lambda DNA and Agarose gel elektrophoresis - Principle of the experiment
4 important questions on Restriction digest of phage lambda DNA and Agarose gel elektrophoresis - Principle of the experiment
The phage λ DNA is subjected to a restriction breakdown or restriction digest. What are the preparation steps?
- Prepare a mixture of phage λ DNA, restriction enzyme(s) and a buffer at which the restriction enzyme is optimally active.
- Subject the phage λ DNA with the restriction enzyme at its optimal temperature.
Why do some enzymes need the addition of Bovine Serum Albumin (BSA)
Why are restriction enzymes stored at -20°C ?
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What is required to obtain an optimal enzymatic activity?
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