RNA isolation and quality control - RNA isolation - RNases

6 important questions on RNA isolation and quality control - RNA isolation - RNases

RNases can be devided into two groups? Which ones?

  1. Endoribonucleases
  2. Exoribonucleases

Where does the human body use RNases for?

To defend against invading microorganisms by secreting these enzymes in fluids such as tears, saliva and mucus.

What is the primary source of RNases within most environments

Microorganisms such as bacteria and fungi
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RNases are fairly small, compact proteins containing several cysteine residues that form numerous intramolecular disulfide bonds.  This results in ?

Denatured RNases that tend to regain their native structure and partial function after being cooled to room temperature in the abscence of a denaturant.

When working with RNA some basic precautions need to be taken.

Wearing gloves and use of RNase-free chemicals and reagents.

How do you obtain RNase free solutions?

By treament with 0.1% DEPC. DEPC is a strong, but not absolute, inhibitor of RNases and inactivates RNases by covalent modification.

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