Class 4 RNA

11 important questions on Class 4 RNA

What are the two techniques used to investigate RNA expression?

  • Northern Blot
  • Realtime PCR

What makes northern blotting a 'targeted approach'? '

Because you need to have knowledge of the gene related to the studied mRNA.

How is Northern blot standardized?

With a housekeeping gene.
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What is the biggest difference between PCR and qPCR in the starting phase?

The template and SYBR green dye in qPCR.

Why does qPCR use cDNA?

Because the PCR uses DNA polymerase...

What is the function of SYBR green in qPCR?

The double stranded DNA enhances the fluoresence emmision.

Do you need to use a housekeeping gene in all gene expression experiments?

Yes, you need a reference.

What is the difference between quantitive and qualititive measures?

Qualilitive cannot give concrete number discrepancies.

How do northern blot and qPCR fundamentally differ?

  • Source material (RNA vs cDNA)
  • measure (qual vs quan)
  • throughput (low vs high)

What is the use of -omics studies?

Looking and infering about the differences in a population.

Where does the biggest DNA strand end up in the elektrophoresis gel?

Closest to the start.

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