Class 4 RNA
11 important questions on Class 4 RNA
What are the two techniques used to investigate RNA expression?
- Northern Blot
- Realtime PCR
What makes northern blotting a 'targeted approach'? '
How is Northern blot standardized?
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What is the biggest difference between PCR and qPCR in the starting phase?
Why does qPCR use cDNA?
What is the function of SYBR green in qPCR?
Do you need to use a housekeeping gene in all gene expression experiments?
What is the difference between quantitive and qualititive measures?
How do northern blot and qPCR fundamentally differ?
- Source material (RNA vs cDNA)
- measure (qual vs quan)
- throughput (low vs high)
What is the use of -omics studies?
Where does the biggest DNA strand end up in the elektrophoresis gel?
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