Gene Mutations - Detection of Gene Mutations
8 important questions on Gene Mutations - Detection of Gene Mutations
Hoe kunnen de resultaten op een Denaturing Gradient Gel Electrophoresis (DGGE) gel worden geïnterpreteerd?
- De snelheid van migratie door de gel zegt iets over de relatieve verhouding in AT en CG verbindingen. AT migreert sneller door de gel omdat dit sneller denatureert dan CG.
- Twee bandjes kan duiden op de aanwezigheid van twee verschillende allelen voor een bepaalde locatie. Dit geeft genetische variatie weer op die specifieke locatie. Het kan het gevolg zijn van een SNP.
Wat is ASO dot blot?
- Vertrouwt op bindende effecten van nucleotide-mismatches.
- Monster in oplossing wordt gespot op nitrocellulose.
- Gelabelde oligonucleotideprobe wordt gehybridiseerd met geïmmobiliseerd monster.
- Drie monsters worden gespot op duplicate membranen: één membraan is blootgesteld aan de probe die complementair is aan de WT-sequentie (wt probe) en één membraan is blootgesteld aan de probe die complementair is aan de mutant-sequentie (m probe)
- Kan SNP detecteren
Wat is het verschil tussen forward dot blot en reverse dot blot?
- Forward dot blot: de specifieke probes worden op het membraan geïmmobiliseerd en het geteste DNA wordt geïncubeerd met het membraan.
- Reverse dot blot: de DNA-sequenties worden geïmmobiliseerd op het membraan, vervolgens worden de specifieke probes geïncubeerd met het membraan. (met multiplex-PCR kunnen meerdere mutaties tegelijk worden getest)
Reverse: membraan is drager van DNA (monster gespot op membraan en gelabeld)
- Higher grades + faster learning
- Never study anything twice
- 100% sure, 100% understanding
For my 1st exam I got a C. Then, I started looking for ways to learn better and I came across Study Smart. Since then I have scored a A+, A, A- and another A. I highly recommend it to everyone who wants to hear it. I am so happy with it!!
I was struggling to finish all my first-year subjects for 3 years. Then I discovered Study Smart, which helped me to finish all of them within 3 months.
Because of StudySmart, things are going well in many ways. Before, I always had study stress and no time for anything. Now I feel calm and confident (because of the program). I used to score a B, now I usually score an A or A+.
Hey Chris, I really want to thank you very much for developing the platform, I usually got around C for my exams, now I scored an A+, an A and a A- !!
Since using Study Smart, my average has increased significantly. I even completed a statistics course with a A+ while I'm bad at statistics. I took on extra courses this year, because of Study Smart. Thanks so much!
Ever since I started studying with StudySmart I passed all my exams !!! No more re-sits!! At first, I was happy with a pass and now I only get good grades. Many thanks!
I aced all the courses I studied with Study Smart. For law, I first scored a D, and now an A! I’m 100% sure that I’ll pass all the courses I study with Study Smart.
I always thought I studied well. My grades were fine. I could never have imagined that applying these study skills would have such a huge and direct impact on my grades, my speed and the pleasure I have in learning.
For the first time, I finally feel totally confident about the way I learn. I have the perfect overview and make great progress in terms of speed and the grades I get. Thanks, Chris!
I immediately started to enjoy learning again, and my grades soared. All of a sudden, everything went better. I highly recommend Study Smart to all students..
Being able to create my materials online made the process of studying so much smoother and quicker. I no longer have to spend a huge amount of my time on creating my summaries.
At first, my notes were a mess. Now I use Study Smart to take my notes in a perfectly structured way. It helps me to save a lot of time and do other things I enjoy.
Students that study with Study Smart get better grades. The system applies all the learning methods in such a way that both well-performing students and students who struggle perform much better.
Hi Chris, I would like to thank you very much. Last year I scored C on a course, and this year I got an A+ thanks to your method! Thank you very much!
The largest effect is that my child is more enthusiastic about school! Every parent wants their children to be happy at school, Study Smart made it happen.
In high school, I failed several exams, and that has really changed now. I actually needed this tool very much back then. Since I started studying with StudySmart, I haven't had any D's. My average score is now an A and those are results I would have never had in the past
With my old method, I passed only 3 out of 8 courses. Since I started taking my notes digitally in Study Smart, I have passed all my courses on the first try. For me, StudySmart takes away the stress of wondering if I will pass or not.
Thanks to StudySmart, I passed all my exams, and with better grades than before! On top of that, I have mastered a very good study method now, which I am confident will help me earn my degree.
Wat is de vergelijking van dot blot, reverse dot blot en arrays?
- Dot blot - gespotte probes
- Reverse dot blot - gespot DNA
- Array - geordend patroon van probes op een vaste oppervlakte.
Wat is nuclease cleavage als detectiemethode van puntmutaties?
- Nucleasen knippen ss-bubbels in heteroduplexen
- Regio van interesse: geamplificeerd door PCR
- PCR-product wordt gedenatureerd en renatureerd met of zonder toegevoegd normaal PCR-product
- Gerenatureerde duplexen worden gedigesteerd met nuclease
- Producten worden waargenomen met gelelektroforese
- Geknipte fragmenten geven aanwezigheid van mutatie weer
Wat is nonisotopic RNase cleavage assay (NIRCA)?
Wat zijn de stappen in Nuclease Cleavage?
- Nucleasen die enkelstrengige bellen in heteroduplexen knippen.
- PCR
- PCR-product wordt gedenatureerd en gerenatureerd met of zonder toegevoegd normaal PCR-product.
- Gerenatureerde duplexen worden gedigereerd met nuclease, bijvoorbeeld S1-nuclease.
- Gelelektroforese: geknipte fragmenten tonen de aanwezigheid van mutatie aan.
Wat is invader technology als een cleavage based methode voor de detectie van puntmutaties?
- Mutatiedetectie met Cleavase-enzym (Flap endonuclease I) (herkent de overlappende structuur en knipt de 5'-Flap aan de primaire probe)
- Monster wordt gemengd met probes en enzym.
- Enzymsplitsing van de probe-testmonsterhybride zal een fluorescerend signaal opleveren.
- Er treedt alleen een signaal op als de volgorde van de probe en het testmonster complementair zijn.
The question on the page originate from the summary of the following study material:
- A unique study and practice tool
- Never study anything twice again
- Get the grades you hope for
- 100% sure, 100% understanding
