Are multiple cell states a general property of cancer?
5 important questions on Are multiple cell states a general property of cancer?
How does single cell RNA sequencing work in cancer analysis?
- From solid tissue, single cell are isolated
- RNA from it is taken and cDNA is made which are amplified
- form the single cell RNA sequencing, look at the single cel RNA expression and identify the uniek transcription states
- Identify cell type
- See difference between cancer and non cancer cell
What is the application of single cell RNAseq?
- Deconvolving heterogeneity cell population
- Trajectory analysis of cell state transition
- Network interference
What are the 3 major cellular subtypes in oligodendroglioma? And which type tumor do they have?
- Stem-like (cycling cells)
- astro-like (AC)
- oligo-like (OC)
- (undifferentated)
Developmental hierarchy of these tumor types can differ across tumor.
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What are the two transcriptional states in melanoma?
- AXL - mesenchymal type cells
- MITF - lineage committed cells
Each melanoma includes two different cell types: ALX+ enriches during RAF/ERK inhibition treatment
What is the drug tolerant cell type in melanoma?
- Trametinib ( MEK inhibitor)
- RXR antagonist
combination of drug kills all cells
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