Neurobiological research - Different models of bevioral research - Mouse
8 important questions on Neurobiological research - Different models of bevioral research - Mouse
What are knock-out mice?
What are three ways to create a knockout mouse?
- Using Crispr-Cas9 for genetic adaptations
- Cre-LoxP system
How is a knockout mouse made with the use of embryonic stem cells?
2. Therefore, there will be a mouse that have cells from knockout cell line and some from the healthy mouse.
3. This will be mated with a wild type of mouse and the offspring will be black or white. The black mouse has a homozygous knockout gene.
4. Those mice are then paired together and therefore 25% of the offspring will be homozygous dominant for the knock-out gene.
5. Determined Using PCR
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Where is the cre-Lox system used for in research?
How does the Cre-Lox system work?
How does the LoxP sited needs to be directed to result in a knock-out?
What is the difference between homologous vs site-specific recombination?
What is Conditional knock out?
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