Drosophila GSC
28 important questions on Drosophila GSC
What location is important for Drosophila GSCs?
Where are female drosophila GSCs located?
Three types of stem cells in Dros. Ovarium
2. Escort stem cells: surround the oocyte
3. Follicle stem cells, become follicle cells
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What cells create dros GSC niche?
What is Dpp + pathway summary
Signal that induces GSC fate in the niche
Gets secreted -> binds heterodimer receptor, that autophosphorylates > receptor phosphorylates Smad, that functions as a TF
What does active Smad do?
What is the Bam mutant phenotype?
How does GSC get dpp signal?
What is the function of Bam?
What do you know about dpp gradient and what is its effect
By what cell types is Dpp expression regulated
How do terminal filament cells regulate Dpp expression by cap cells?
It dimerises the Domeless receptor, where JAK can bind to phosphorylate STAT. STAT forms a dimer and functions as a transcription factor, increasing Dpp transcription
What is the definition of ectopic expression
What happens in the first stages of oocyte differentiation
How does the connection between nurse cells and the oocytes look
What is transported through the ring canals and how does this transport work
What happens when all maternal RNA is in the oocyte
What do the male reproductional organs look like
Which cell is the niche in males
What is the function of cyst cells (males)
What happens at the beginning of male GSC differentiation
What factor is secreted by the Hub cells
What is the difference of JAK/STAT effect between GSCs and CySCs
Its effect in GSCs is that it is necessary for E-cadherin molecules, so the GSC can remain bound to the Hub
What factor regulates self-renewal in male GSCs?
Expression of Zfh-1 in CySCs leads to Dpp expression by these cells, activating its downstream effectors in the GSC. GSC also gets this signal from the Hub cells
Why is E-cadherin loss problematic
How is the gradient of Unpaired in males
What is the role of cyst cells around the Gb
2 versions of restoration of GSC population, how do they work
2. Reversion: differentiated cells go back to stem cells, this can happen. Even Hub cells can revert and become GSCs to re-establish a GSC population
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