Spectrophotometry UV-Vis
13 important questions on Spectrophotometry UV-Vis
What is the definition of Spectrometry/Spectrophotometry?
•Intensity of radiation (light) is measured and converted to an electronic signal (that we then can interput to gather information about the compoud of interes)
•Measurement of absorbed light (absorbance) and emitted light (emission)
So we can measure light that is absorbed by the ucompound or study light which is emitted (Uitgezonden/mab3ooth) by the compound.
What is the information that we gain with spectrophotometry?
- The amount of compound in the sample that we are studying
What are the properties of light fields?
Magnetic field = Horizontal
(MF is oriented 90 degrees to EF)
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What is a Wavelength? Give also the unit
Measured in (greek letter: Lambda)
Electrical Field
What is an Amplitude ? Give also the unit
Electrical FIeld
What is Frequency? Give also the unit
What is the speed of light propagation (voortplanting/intishaar) formula? Also explain what the parameters mean
•Speed of light propagation: vi = vλi
•vi changes from one medium to the next
•n is constant (defined by the light source)
•Thus, if speed changes, l changes
In Glass (comparable to air)
vi = Speed of light propagation (Changes)
v = Frequency of the light (Remains constant in different medium )
λi = Wavelength (Shorter)
What is the range of wavelengths of the electromagnetic spectrum?
What is wavelength range of Ultraviolet light?
What is the wavelength range of Visible light?
Speed of light in air in constant, how fast is it?
What is the relationship between frequency and wavelength of an electromagnetic wave, i ?
vi = vλi
What value of absorbance corresponds to 45.0% T ? (b) If a 0.010 0 M solution exhibits 45.0% T at some wavelength, what will be the percent transmittance for a 0.020 0 M solution of the same substance?
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